【貨號】 BP-DL021

【規格】 7×50mL/7×100mL/7×500mL

【保存】 10~30℃，避光，12個(gè)月有效。

【產(chǎn)品組成】

 

【產(chǎn)品簡(jiǎn)介】

結締組織狹義上是指其含有的三種纖維：膠原纖維、網(wǎng)狀纖維、彈力纖維，而膠原纖維（collagen fiber）是分布最廣、含量最多的一種纖維。Masson三色染色又稱(chēng)馬松染色，是結締組織染色中最經(jīng)典的一種方法，是膠原纖維染色權威而經(jīng)典的技術(shù)方法。所謂三色染色通常是指染胞核和能選擇性的顯示膠原纖維和肌纖維。該法染色原理與陰離子染料分子的大小和組織的滲透有關(guān)：分子的大小由分子量來(lái)體現，小分子量易穿透結構致密、滲透性低的組織；而大分子量則只能進(jìn)入結構疏松的、滲透性高的組織。然而，淡綠或苯胺藍的分子量都很大，因此Masson染色后肌纖維呈紅色，膠原纖維呈綠色（淡綠）或藍色（苯胺藍），主要用于區分膠原纖維和肌纖維。染色穩定；分化時(shí)間短，1~2s；色彩清晰鮮艷；適用范圍廣，適宜于組織的石蠟切片、冰凍切片等染色；所染切片保存時(shí)間長(cháng)且不易褪色。

【使用方法】 

1、切片常規脫蠟至水，用配制好的Weigert鐵蘇木素染色5~10min。

2、用酸性乙醇分化液分化，水洗。

3、用Masson藍化液返藍，水洗。

4、蒸餾水洗1min。

5、麗春紅品紅染色液染色5~10min（鏡下控制染色時(shí)間）。

6、蒸餾水稍洗。

7、磷鉬酸溶液洗5~10min。

8、直接入苯胺藍染色液中染色1~2min。

9、弱酸溶液洗1min。

10、95%乙醇快速脫水。無(wú)水乙醇脫水3次，每次5~10s。

11、二甲苯透明3次，每次1~2min。中性樹(shù)膠封固。

【染色結果】

 

【注意事項】

1、切片脫蠟應盡量干凈。固定起著(zhù)重要的作用，使用不同的固定液可延或縮短染色時(shí)間。

2、取A1、A2等量混合即為Weigert鐵蘇木素染液，一般24h失去染色力。

3、酸性乙醇分化時(shí)間應根據切片厚薄、組織的類(lèi)別和新舊而定。

4、弱酸溶液可使色彩更清晰鮮艷，如使用量大可自行配制0.1~0.3%乙酸溶液予以替代。

5、磷鉬酸分化時(shí)要在鏡下控制，分化到膠原纖維呈淡紅色、纖維呈紅色即可。分化時(shí)間根據染色深淺而定，一般1~2min。

6、Masson藍化液亦可自行配制Scott促藍液或0.1~1%碳酸鋰水溶液予以替代。

 

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